Table 3 Statistical analysis of the effects of Plackett–Burman design on PEN-G biotransformation efficiency (%)
Variables | Coefficients | t value | Main Effect | p value | Confidence %)) |
|---|---|---|---|---|---|
| Â | 72.432 | 300.710 | Â | 0.002 | 99.8 |
Yeast extract (g/l) | 8.230 | 32.828 | 16.460 | 0.019 | 98.1 |
PEN-G (g/l) | 6.479 | 25.843 | 12.958 | 0.025 | 97.5 |
Glucose (g/l) | -1.185 | -4.725 | -2.369 | 0.133 | 86.7 |
K2HPO4 (g/l) | 1.315 | 5.243 | 2.629 | 0.120 | 88.0 |
KH2PO4 (g/l) | 7.103 | 28.332 | 14.206 | 0.022 | 97.8 |
MgSO4.7H2O (g/l) | 7.403 | 29.527 | 14.805 | 0.022 | 97.8 |
Trace elements (%) | 4.017 | 16.021 | 8.033 | 0.040 | 96.0 |
Inoculum size (%) | 2.593 | 10.342 | 5.186 | 0.061 | 93.9 |
pH | 2.710 | 10.810 | 5.420 | 0.059 | 94.1 |
Temperature (oC) | 1.253 | 4.997 | 2.506 | 0.126 | 87.4 |
Time of incubation (h) | -5.974 | -23.829 | -11.948 | 0.027 | 97.3 |