Fig. 2

a Extraction and purification of IgY antibody. Extracted IgY (L1), purified IgY (L2–L5), and L6 marker. b Reactivity of IgY by Western blot. L1: native SEB, L2: denatured SEB, and L3: marker. c Biotinylation of anti SEB monoclonal antibody (P1–P5) various elution of monoclonal antibody, P6: non-biotinylated antibody, (P7, P8): control